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Interaction between Yersinia pseudotuberculosis and Y. enterocolitica and fish cells
N F Timchenko1, V A Zahaleva, L P Xodakova
1Institute of Epidemiology and Microbiology, Academy of Medical Sciences, Vladivostok, Russia.
Microbios
|January 1, 1994
Summary
Virulent Yersinia bacteria, when infecting fish cells at 25°C, adhere to cell surfaces, are ingested, and exhibit cytotoxicity. Intracellular Yersinia divide within phagosomes but do not enter the nucleus.
Area of Science:
- Microbiology
- Cell Biology
- Fish Immunology
Background:
- Yersinia pseudotuberculosis and Yersinia enterocolitica are significant bacterial pathogens.
- Understanding Yersinia interactions with host cells is crucial for fish health.
Purpose of the Study:
- To investigate the interaction between virulent Yersinia strains and cultured fish cells.
- To characterize the cellular response and bacterial behavior during infection.
Main Methods:
- Culturing virulent Yersinia strains (Y. pseudotuberculosis, Y. enterocolitica) at 10°C.
- Infecting cultured fish cells with Yersinia at 25°C.
- Microscopic observation of bacterial-cell interactions, including adherence, ingestion, and intracellular fate.
Main Results:
- Yersinia infection at 25°C induced fish cell pseudopodia formation and bacterial adherence.
- Bacteria were ingested by cells approximately 2 hours post-infection.
- Yersinia demonstrated cytotoxicity towards fish cells, with intracellular bacteria observed dividing within phagosomes but not in the nucleus.
Conclusions:
- Yersinia species exhibit pathogenic mechanisms including adherence, invasion, and cytotoxicity in fish cell models.
- Intracellular Yersinia survive and replicate within phagosomes, indicating potential for intracellular pathogenesis.
- The study highlights the importance of temperature in modulating Yersinia virulence and host cell interactions.