Primary sequence of a putative pyrophosphate-linked phosphofructokinase gene of Giardia lamblia

Based on sequence information from a cosmid library, we obtained a genomic clone that encodes a putative inorganic pyrophosphate (PPi)-linked phosphofructokinase of Giardia lamblia. The open reading frame specified a protein of 59.8 kDa homologous to PPi- and ATP-linked phosphofructokinases. The greatest similarity (over 50% positional identity) was noted to the beta-subunits of both the Solanum tuberosum (potato) and Ricinus communis (castor bean) PPi-phosphofructokinases.