Cell growth on immobilized cell-growth factor. 10. Insulin and polyallylamine co-immobilized materials

Polyallylamine, which is cationic and enhances cell adhesion electrostatically (non-specifically), was co-immobilized with a cell growth factor, insulin, onto a surface-hydrolysed poly(methyl methacrylate) membrane. The adhesion of mouse STO fibroblast cells was accelerated by the immobilization of polyallylamine. The adhesion and growth of the cells were both markedly accelerated by the co-immobilization of the polyallylamine and insulin. The co-immobilized membrane can be applied for a new technique for protein-free cell culture.