[Effects of calcitonin and indomethacin on bone resorption mediated by interleukin-1]

The effects of calcitonin and indomethacin on bone resorption stimulated by interleukin-1 (IL-1) were studied in cultured neonatal mouse calvariae. The results showed that calcium release from cultured calvariae was significantly increased after adding 20 ng/ml IL-1 (P < 0.01). The increase of calcium release stimulated by IL-1 was blocked by both calcitonin at the concentration of 10, 100, 1,000 ng/ml and indomethacin (10(-6), 10(-5) mol/L). The stimulation of calcium release by IL-1 was also blocked even adding 10 mol/L indomethacin 5 hours after adding IL-1 into the culture. These data showed that both calcitonin and indomethacin can inhibit bone resorption stimulated by IL-1. Thus, it is possible that calcitonin and indomethacin may be used as a therapeutic agent to block inflammatory bone resorption mediated by IL-1.