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CD4+V beta 8+ T cells mediate herpes stromal keratitis
A Heiligenhaus1, A Berra, C S Foster
1Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, MA 02114.
Current Eye Research
|October 1, 1994
Summary
Herpes simplex stromal keratitis (HSK) involves T lymphocytes. Researchers found that CD4+V beta 8+ T cells infiltrate the corneas of susceptible mice, indicating their role in HSK.
Area of Science:
- Immunology
- Ophthalmology
- Virology
Background:
- T lymphocytes are crucial for the immune response in herpes simplex stromal keratitis (HSK).
- Understanding T cell subsets and their receptor usage is key to HSK pathogenesis.
Purpose of the Study:
- To investigate T cell subsets and T cell receptor variable beta (TCR V beta) repertoire in the eye during HSV-induced keratitis.
- To explore differential TCR V beta usage in HSK-resistant versus susceptible mouse models.
Main Methods:
- Immunohistologic analysis of corneal tissue from HSV-infected mice.
- Comparison of T cell infiltration and TCR V beta expression in susceptible (C.AL-20) and resistant (C.B-17) congenic mouse strains.
Main Results:
- Susceptible C.AL-20 mice showed significant infiltration of CD4+ and V beta 8 expressing T cells in inflamed corneas.
- Resistant C.B-17 mice did not exhibit this specific T cell infiltration pattern.
Conclusions:
- CD4+V beta 8+ T cells are implicated in mediating the pathogenesis of HSV-1 stromal keratitis.
- Differential T cell subset involvement may contribute to varying HSK susceptibility.