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Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling
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Development of an 125I-postlabeling assay as a simple, rapid, and sensitive index of DNA-protein cross-links

Z Zhuang1, M Costa

  • 1Nelson Institute of Environmental Medicine, New York University Medical Center, New York 10016.

Environmental Health Perspectives
|September 1, 1994
PubMed

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...