Related Experiment Videos
Effect of aluminium (Al) on brain mitochondrial monoamine oxidase-A (MAO-A) activity--an in vitro kinetic study
1Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Mysore, India.
Molecular and Cellular Biochemistry
|August 17, 1994
Abstract:
Effect of aluminium (Al) on rat brain mitochondrial monoamine oxidase-A (MAO-A) was studied in vitro at three different pH (4.0, 7.4 and 9.0) values. The results have shown that Al is a non-competitive inhibitor for MAO-A. The data also showed that MAO-A inhibition by Al varies with free Al3+ concentration and different forms of Al under different pH conditions. Al altered the maximum velocity (Vmax) of MAO-A but did not affect substrate-enzyme affinity (Km). Al formed a strong chelation with the substrate (Kynuramine) (1:1).