Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Chain condensation in protein folding

H M Chen1, T Y Tsong

  • 1Department of Biochemistry, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon.

Biochimie
|January 1, 1994
PubMed
Summary

Staphylococcal nuclease folding involves three kinetic reactions. The tau 2 reaction, linked to chain condensation, aligns with a diffusion/collision/coalescence model, suggesting electrostatic interactions guide protein folding.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Clinicopathological characteristics and prognosis of lupus nephritis patients with acute kidney disease].

Zhonghua nei ke za zhi·2026
Same author

[Analysis of death reporting omissions among permanent residents in Beijing from 2017 to 2021 based on the capture-recapture method].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2026
Same author

[Influencing factors analysis and prediction model establishment of toe-amputation in patients with diabetic foot].

Zhonghua yi xue za zhi·2025
Same author

[Maternal and Child Health Exhibition in the early days of the People's Republic of China].

Zhonghua yi shi za zhi (Beijing, China : 1980)·2024
Same author

[Advances in the study of postpancreatectomy acute pancreatitis].

Zhonghua wai ke za zhi [Chinese journal of surgery]·2023
Same author

[Clinicopathological and molecular genetic characterization of colorectal angiosarcoma].

Zhonghua bing li xue za zhi = Chinese journal of pathology·2023

Area of Science:

  • Biochemistry
  • Protein Folding Dynamics
  • Enzymology

Background:

  • Staphylococcal nuclease is a model protein for studying folding mechanisms.
  • Acid denaturation unfolds the protein, allowing refolding studies.
  • Previous work identified multiple kinetic phases in protein refolding.

Purpose of the Study:

  • To characterize the kinetic reactions involved in the refolding of acid-denatured staphylococcal nuclease.
  • To investigate the role of solution viscosity and specific amino acid residues in the folding process.

Main Methods:

  • Stopped-flow kinetic method to resolve refolding reactions.
  • Viscosity dependence studies to probe reaction mechanisms.
  • Site-directed mutagenesis to identify key residues involved in folding.

Main Results:

  • Three distinct kinetic reactions with relaxation times tau 1, tau 2, and tau 3 were identified.
  • The tau 2 reaction exhibited viscosity dependence consistent with the diffusion/collision/coalescence model.
  • Mutagenesis suggested electrostatic interactions involving Glu75, His121, and Lys9 are crucial for chain condensation.

Conclusions:

  • Protein folding of staphylococcal nuclease is a multi-step process.
  • Chain condensation, a key folding event, is likely driven by specific electrostatic interactions.
  • The diffusion/collision/coalescence model provides a framework for understanding the tau 2 folding phase.

Related Experiment Videos