Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Differentiation and patterning in Dictyostelium

R R Kay1

  • 1MRC Laboratory of Molecular Biology, Cambridge, UK.

Current Opinion in Genetics & Development
|October 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The genome of the social amoeba Dictyostelium discoideum.

Nature·2005
Same author

Cross-induction of cell types in Dictyostelium: evidence that DIF-1 is made by prespore cells.

Development (Cambridge, England)·2001
Same author

The role of DIF-1 signaling in Dictyostelium development.

Molecular cell·2001
Same author

Cell-fate choice in Dictyostelium: intrinsic biases modulate sensitivity to DIF signaling.

Developmental biology·2000
Same author

Ca(2+) signalling is not required for chemotaxis in Dictyostelium.

The EMBO journal·2000
Same author

Development at the edge of multi-cellularity: Dictyostelium discoideum.

The International journal of developmental biology·2000
Same journal

Temporal trajectories underlying adult neuronal diversity.

Current opinion in genetics & development·2026
Same journal

Transcription regulation of cell fate plasticity - from embryonic development to tissue regeneration.

Current opinion in genetics & development·2026
Same journal

Shared molecular and cellular programs during regeneration of glandular epithelia.

Current opinion in genetics & development·2026
Same journal

Lineage tracing in human cortical development.

Current opinion in genetics & development·2026
Same journal

Cis-regulatory strategies in developmental patterning.

Current opinion in genetics & development·2026
Same journal

GABAergic neuron fate specification and lineage allocation: from development to disorder.

Current opinion in genetics & development·2026
See all related articles

Dictyostelium development relies on chemical signals like cAMP and ammonia. Researchers identified a novel protein essential for activating adenylyl cyclase, advancing understanding of this slime mold

Area of Science:

  • Cellular and developmental biology
  • Molecular biology
  • Biochemistry

Background:

  • Dictyostelium discoideum development is a model system for studying cell differentiation and multicellularity.
  • Development is regulated by diffusible signaling molecules, including cyclic adenosine monophosphate (cAMP) and differentiation-inducing factor (DIF).
  • Understanding the molecular mechanisms underlying signal transduction and gene regulation is crucial.

Purpose of the Study:

  • To investigate the molecular basis of Dictyostelium development.
  • To identify novel genes and proteins involved in signal transduction pathways.
  • To elucidate the function of newly discovered proteins in developmental processes.

Main Methods:

  • Utilized restriction enzyme mediated integration (REMI) insertional mutagenesis for gene discovery.

Related Experiment Videos

  • Employed molecular cloning techniques to isolate and characterize genes.
  • Investigated protein function through biochemical assays, including adenylyl cyclase activation.

    • Main Results:

    • Successfully cloned the first genes in Dictyostelium using REMI mutagenesis.
    • Identified a novel gene encoding a cytosolic protein crucial for adenylyl cyclase activation.
    • Demonstrated the essential role of this protein in regulating cAMP signaling pathways.

    Conclusions:

    • The discovery of this novel protein provides new insights into the regulation of adenylyl cyclase and cAMP signaling in Dictyostelium.
    • This finding contributes to a deeper understanding of the molecular mechanisms governing cellular differentiation during development.
    • Further research on this protein may reveal conserved pathways in other developmental systems.