[Determination of hyaluronidase activity by the rivanol clot method]

A method for hyaluronidase activity measurement in various biologic samples is suggested, based on rivanol capacity to form a clot with hyaluronic acid inversely proportional to this acid depolymerization under the effect of hyaluronidase. This method sensitivity is ten times higher than that of the mucin clot method; any colorimetric or fluorometric method can be used for detection. The method has been adapted to measurements of commercial hyaluronidase preparations and of blood serum hyaluronidase activity.