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A semi-micro method for fibrinogen determination essentially unaffected by turbidity
W E Carroll1, R D Jackson, D J Herring
1Department of Pathology, Santa Barbara Cottage Hospital, CA 93105.
Research Communications in Molecular Pathology and Pharmacology
|October 1, 1994
Summary
A new BR BLUE method accurately determines fibrinogen levels in turbid samples. This Coomassie Brilliant Blue G-250 assay shows excellent correlation with established methods, offering a precise and linear alternative.
Area of Science:
- Clinical Chemistry
- Hematology
- Biochemical Assays
Background:
- Accurate fibrinogen determination is crucial for hemostasis assessment.
- Turbid specimens pose challenges for traditional coagulation assays.
- Need for reliable fibrinogen measurement methods in clinical diagnostics.
Purpose of the Study:
- To introduce and evaluate the BR BLUE semi-micro method for fibrinogen determination.
- To assess the performance of BR BLUE in potentially turbid biological samples.
- To compare the BR BLUE method with established coagulation testing techniques.
Main Methods:
- Development of a semi-micro assay using Coomassie Brilliant Blue G-250 (BR BLUE).
- Comparison with the Clauss method using automated (MLA Electra 1000C) and semi-automated (Mechrolab Clot Timer) instruments.
- Analysis of correlation, precision, and linearity of the BR BLUE method.
Main Results:
- Excellent correlation (r = 0.97) between the BR BLUE method and reference methods.
- The BR BLUE method demonstrated good precision.
- Linearity of the BR BLUE method was confirmed for fibrinogen quantification.
Conclusions:
- The BR BLUE method is a viable and accurate technique for fibrinogen determination, even in turbid samples.
- This assay offers a reliable alternative to existing methods for clinical laboratories.
- The method's precision and linearity support its use in routine diagnostics.