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Related Experiment Videos

Antibody penetration into LR-White sections

S H Brorson1, N Roos, F Skjørten

  • 1Department of Pathology, Ullevål Hospital, Oslo, Norway.

Micron (Oxford, England : 1993)
|January 1, 1994
PubMed
Summary
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Antibodies cannot penetrate LR-WHITE resin sections, preventing antigen demonstration in post-embedding immunoelectron microscopy. This finding supports LR-WHITE

Area of Science:

  • Immunoelectron microscopy
  • Biochemistry
  • Materials Science

Background:

  • LR-WHITE resin is commonly used for embedding biological samples.
  • Post-embedding techniques are crucial for antigen localization in electron microscopy.
  • Previous studies suggested antibody penetration into LR-WHITE sections.

Purpose of the Study:

  • To investigate the penetration of antibodies into LR-WHITE resin sections.
  • To evaluate the suitability of LR-WHITE for immunoelectron microscopy applications.

Main Methods:

  • Reembedding and tilting of immunogold-labeled LR-WHITE sections.
  • Immunolabeling of cylindrical structures and tissues (fibrin, pituitary) embedded in LR-WHITE.
  • Application of antibodies on opposite sides of ultrathin sections.

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Main Results:

  • No evidence of antibody penetration into the interior of LR-WHITE sections was observed.
  • Results contradicted previous findings on antibody penetration.
  • LR-WHITE resin demonstrated resistance to antibody diffusion.

Conclusions:

  • Antigens cannot be demonstrated within LR-WHITE sections using post-embedding techniques.
  • LR-WHITE resin is suitable for quantitative immunoelectron microscopy.
  • LR-WHITE resin enables safe double immunogold labeling due to limited immunoreagent diffusion.