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Related Experiment Videos

A recombination-dependent replicating instability in Salmonella typhimurium

G E Murray, P F Smith-Keary

    Mutation Research
    |September 1, 1976
    PubMed
    Summary

    Salmonella typhimurium mutants exhibit unstable tryptophan requirements linked to gene duplication. This instability requires a functional rec+ gene for genetic recombination.

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    Applied and environmental microbiology·1984

    Area of Science:

    • Microbiology
    • Bacterial Genetics
    • Molecular Biology

    Background:

    • Investigating auxotrophic mutants in Salmonella typhimurium.
    • Understanding genetic instability in bacterial strains.

    Purpose of the Study:

    • Characterize the unstable tryptophan requirement in Salmonella typhimurium aroD321 mutant.
    • Elucidate the genetic basis of this instability.

    Main Methods:

    • Genetic analysis of Salmonella typhimurium aroD321.
    • Transduction experiments to map genetic loci.
    • Assessing gene dependence on rec+ function.

    Main Results:

    • The aroD321 mutant shows a stable phenylalanine and tyrosine requirement but an unstable tryptophan requirement.
    • Tryptophan-requiring derivatives are unstable and revert.
    • Instability is cotransducible with the aroD region and rec+-dependent.

    Conclusions:

    • The observed instability results from a gene duplication and excision mechanism.
    • This mechanism is dependent on the cell's normal recombinational (rec+) ability.

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