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Expression of scatter factor in human bladder carcinoma
1Department of Radiation Oncology, Long Island Jewish Medical Center, Albert Einstein College of Medicine, New York, N.Y. 10050.
Journal of the National Cancer Institute
|March 1, 1995
Summary
Scatter factor (SF) is elevated in bladder cancer patients, accumulating in tumors and urine. This suggests a potential new marker for urothelial malignancy, possibly due to abnormal urothelial-stromal interactions.
Area of Science:
- Urothelial carcinoma research
- Tumor microenvironment
- Cancer biomarkers
Background:
- Scatter factor (SF) promotes carcinoma cell motility, invasiveness, and angiogenesis.
- Tumor invasion and angiogenesis are hallmarks of aggressive cancers.
- SF accumulation may drive tumor progression to a more malignant phenotype.
Purpose of the Study:
- To determine if SF is overexpressed in bladder carcinoma.
- To investigate potential mechanisms behind SF overproduction in bladder cancer.
Main Methods:
- Measured urinary SF in bladder cancer patients and control groups.
- Assessed SF expression in tumor extracts, tissue sections, and cell lines.
- Utilized ELISA, immunohistochemistry, Western, and Northern blot analyses.
Main Results:
- Urinary SF levels were significantly higher in bladder cancer patients compared to controls.
- High SF levels were found in bladder cancer extracts, particularly in high-grade, invasive tumors.
- Bladder cancer cells produced factors that stimulate SF production by stromal cells.
Conclusions:
- SF is overproduced in bladder carcinomas and accumulates in tumors and urine.
- Abnormal urothelial-stromal interactions may drive SF overproduction.
- SF quantification in urine and tumor warrants further study as a potential marker for urothelial malignancy.