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Determination of doubly labeled water by gas-phase Fourier transform infrared spectroscopy
M A Khaled1, C L Krumdieck, J L Ong
1Department of Nutrition Sciences, University of Alabama at Birmingham.
Metabolism: Clinical and Experimental
|January 1, 1995
Abstract:
Both 2H (deuterium) and 18O (oxygen 18) in isotopically enriched water have been detected by gas-phase Fourier transform infrared (FTIR) spectroscopy at 2,720 and 3,661.8 cm-1, respectively. A linear relationship between varying concentrations of each of these isotopes and their absorbance at the above frequencies indicates that gas-phase FTIR may provide a rapid and potentially less expensive approach to measure doubly labeled water in biological fluids for the estimation of energy expenditure and total body water.