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Cercarial Transformation and in vitro Cultivation of Schistosoma mansoni Schistosomules
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Serodifferentiation of human fascioliasis from schistosomiasis

H Shaheen1, M al Khafif, R M Farag

  • 1US Naval Medical Research Unit #3, Cairo, Egypt.

Tropical and Geographical Medicine
|January 1, 1994
PubMed

A dot enzyme-linked immunosorbent assay (dot-ELISA) was developed using excretory-secretory (ES) antigens of Fasciola gigantica adult worms and freeze-thaw antigen (AFT) of adult Schistosoma mansoni worms. Specific IgG and IgM antibodies were simultaneously investigated in sera from 17 febrile eosinophilic hospitalized patients and 20 healthy controls. Dot-ELISA was shown useful and could differentiate fascioliasis from schistosomiasis prior to parasite egg detection in patient's stool. This assay is field and laboratory applicable.

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