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Updated: Jul 10, 2026
Use of the Protease Fluorescent Detection Kit to Determine Protease Activity
Published on: August 4, 2009
Simultaneous measurement of cytochrome P4501A catalytic activity and total protein concentration with a fluorescence
1Canadian Wildlife Service, National Wildlife Research Centre, Hull, Quebec.
Abstract:
A method for simultaneous measurement of the cytochrome P4501A-associated enzyme, ethoxyresorufin-O-deethylase (EROD), and total protein concentration in liver microsomes is described. EROD assays were carried out in multiwell plates, and the fluorescent product (resorufin) and total proteins were quantified within the same wells with a fluorescence plate reader. Fluorescamine was used for protein assays.
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