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Construction of yeast artificial chromosome libraries by pulsed-field gel electrophoresis
A P Monaco1, Z Larin, H Lehrach
1Human Genetics Laboratory, John Radcliffe Hospital, Headington, Oxford, UK.
Molecular Biotechnology
|June 1, 1994
Abstract:
Yeast artificial chromosome (YAC) libraries have been constructed from a variety of organisms using different approaches. This protocol outlines in detail the construction of YAC libraries with large inserts using size fractionation of partially digested DNA by pulsed-field gel electrophoresis.