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Susceptibility to alloimmunization to platelet HPA-1a antigen involves TAP1 polymorphism
V Braud1, D Chevrier, A Cesbron
1Regional Center for Blood Transfusion, Nantes, France.
Human Immunology
|October 1, 1994
Summary
Alloimmunization against platelet antigen HPA-1a in mothers is linked to HLA class II. TAP1*0102 allele association with NAIT suggests nonclassic HLA class II gene influence on antigen presentation.
Area of Science:
- Immunogenetics
- Molecular Biology
- Neonatal Medicine
Background:
- Alloimmunization against platelet HPA-1a antigen in mothers of thrombocytopenic neonates is strongly associated with HLA class II structures.
- Previous studies identified associations with HLA-DR3, HLA-DR13, and particularly HLA-DR52a.
Purpose of the Study:
- To investigate the role of TAP1 and TAP2 gene polymorphisms in HPA-1a alloimmunization.
- To further characterize the major histocompatibility complex (MHC) genes involved in this immune response.
Main Methods:
- Typing of TAP1 and TAP2 gene polymorphisms using Amplification Refractory Mutation System-Polymerase Chain Reaction (ARMS-PCR).
- Analysis of gene frequencies in women immunized against HPA-1a antigen, stratified by HLA-DR type.
Main Results:
- The TAP1*0102 allele was significantly associated with Neonatal Alloimmune Thrombocytopenia (NAIT) in HLA-DR13-DR52a-immunized women (50%) compared to controls (20%).
- No significant association was found in HLA-DR3-DR52a-immunized women.
- No linkage disequilibrium was observed between TAP1*0102 and DRB1*13 alleles.
Conclusions:
- The TAP1*0102 allele frequency suggests a potential influence of nonclassic HLA class II gene polymorphisms on HPA-1a antigen presentation and recognition.
- Other linked, yet unidentified genes may also play a role in this alloimmunization process.