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Immunochemical quantitation of bromodeoxyuridine: application to cell-cycle kinetics
1Biology and Biotechnology Program, Lawrence Livermore National Laboratory, Livermore, California 94550.
Methods in Cell Biology
|January 1, 1994
Abstract:
We have described several laboratory procedures for the immunochemical staining of the halopyrimidines, BrdUrd and IdUrd, in cell suspensions for flow cytometry and a method for staining histological sections on slides. Halogenated pyrimidine quantitation allows cell-cycle parameters, including total cell-cycle time, phase durations, and growth fraction to be determined. We have presented some flow cytometric data to demonstrate the use of these methods in determining bivariate BrdUrd/DNA histograms with CHO cells and in kinetic studies with the brown Norway rat myeloid leukemia model.