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v-rel Induces ectopic expression of an adhesion molecule, DM-GRASP, during B-lymphoma development
G Zhang1, C Slaughter, E H Humphries
1Mary Babb Randolph Cancer Center, West Virginia University, Morgantown 26506-9177.
Molecular and Cellular Biology
|March 1, 1995
Summary
Researchers identified DM-GRASP as a gene induced by v-rel in avian B-cell tumors. This adhesion molecule
Area of Science:
- Oncology
- Molecular Biology
- Immunology
Background:
- Aberrant gene expression is key in v-rel-induced avian B-cell tumors.
- Identifying these genes aids in understanding tumor development.
Purpose of the Study:
- To identify genes aberrantly expressed in v-rel-induced tumors.
- To characterize the role of DM-GRASP in v-rel-mediated lymphomagenesis.
Main Methods:
- Monoclonal antibody development and immunoprecipitation.
- N-terminal amino acid sequencing and molecular cloning.
- Northern blot analysis and PCR amplification.
Main Results:
- A 120-kDa glycoprotein (p120) was identified and confirmed as DM-GRASP.
- DM-GRASP transcript was detected in v-rel-induced lymphomas and normal tissues like bursa, thymus, and brain.
- Overexpression of v-rel or c-rel induced DM-GRASP expression.
- HY78 antibody inhibited proliferation of v-rel-transformed cells.
Conclusions:
- DM-GRASP is ectopically induced by v-rel during B-cell lymphoma development.
- DM-GRASP may play a role in the growth of v-rel tumor cells.
- Targeting DM-GRASP could be a therapeutic strategy for v-rel-induced tumors.