Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Changes in oncogene mRNA expression during human keratinocyte differentiation

G R Sharpe1, C Fisher, C P Redfern

  • 1University Department of Dermatology, Royal Liverpool University Hospital, UK.

Archives of Dermatological Research
|January 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

N-terminal pro B type natriuretic peptide, but not the new putative cardiac hormone relaxin, predicts prognosis in patients with chronic heart failure.

Heart (British Cardiac Society)·2003
Same author

Transcardiac and transpulmonary gradients in the putative new cardiovascular hormone relaxin.

Heart (British Cardiac Society)·2003
Same author

Calponin and h-caldesmon expression in synovial sarcoma; the use of calponin in diagnosis.

Histopathology·2003
Same author

Tumour thickness predicts cervical nodal metastases and survival in early oral tongue cancer.

Oral oncology·2003
Same author

Hb Q-India: an uncommon variant diagnosed in three Punjabi patients with diabetes is identified by a novel DNA analysis test.

Journal of clinical pathology·2003
Same author

Imaging features of aggressive angiomyxoma.

Clinical radiology·2003

Proto-oncogene expression, specifically c-myc and c-jun, is altered during keratinocyte differentiation. Calcium-induced differentiation significantly decreases c-myc RNA levels, while H-ras expression remains unchanged.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Oncology

Background:

  • Nuclear proto-oncogenes regulate cell proliferation and differentiation.
  • Understanding proto-oncogene expression is crucial for controlling the balance between these processes.

Purpose of the Study:

  • To investigate the role of proto-oncogene expression changes in cultured human keratinocytes during differentiation.
  • To analyze the impact of various differentiation-inducing conditions on c-myc, c-jun, and H-ras RNA levels.

Main Methods:

  • Cultured human keratinocytes were subjected to differentiation stimuli (calcium, TPA, TGF-beta, retinoic acid).
  • Cellular RNA was extracted, size-fractionated, and hybridized with labeled probes for c-myc, c-jun, H-ras, beta-actin, and ribosomal 18s RNA.
  • Quantitative analysis of RNA levels was performed using Northern blotting techniques.

Related Experiment Videos

Main Results:

  • Increasing extracellular calcium concentration significantly decreased c-myc RNA levels within 8 hours, with partial recovery over 48 hours.
  • c-jun RNA levels showed a gradual reduction during differentiation.
  • Treatment with TPA also reduced c-myc RNA, while TGF-beta and retinoic acid did not alter c-myc levels.
  • H-ras RNA levels remained unaffected by all tested differentiation conditions.

Conclusions:

  • Proto-oncogene expression, particularly c-myc, is dynamically regulated during keratinocyte differentiation.
  • Specific signaling pathways, like calcium-mediated differentiation, lead to distinct modulation of proto-oncogene expression.
  • These findings contribute to understanding the molecular mechanisms governing keratinocyte fate decisions.