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Early decrease in GLUT4 protein levels in brown adipose tissue of New Zealand obese mice

L Ferreras1, A S Kelada, M McCoy

  • 1Department of Medicine (Royal Melbourne Hospital), University of Melbourne, Parkville, Australia.

International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity
|November 1, 1994
PubMed
Summary

New Zealand Obese (NZO) mice show reduced glucose transporter type 4 (GLUT4) protein in most tissues by adulthood. This decrease in GLUT4 occurs early in brown adipose tissue (BAT) of these obese mice.

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Area of Science:

  • Metabolic research
  • Obesity studies
  • Molecular biology

Background:

  • Insulin resistance is a hallmark of obesity.
  • The New Zealand Obese (NZO) mouse model exhibits insulin resistance.
  • The role of glucose transporter type 4 (GLUT4) in this model requires clarification.

Purpose of the Study:

  • To investigate if insulin resistance in NZO mice correlates with decreased GLUT4 protein levels.
  • To determine the timing of these potential GLUT4 changes during the development of obesity.

Main Methods:

  • Western blotting was used to quantify GLUT4 protein levels.
  • Tissues were analyzed from 4- and 20-week-old NZO and control NZC mice.
  • Specific antibodies targeted the C-terminal end of GLUT4.

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Main Results:

  • Adult (20-week-old) NZO mice had lower GLUT4 in adipose tissue, heart, diaphragm, and leg muscles compared to controls.
  • At 4 weeks, GLUT4 was significantly reduced (52%) in brown adipose tissue (BAT) of NZO mice.
  • GLUT4 levels were not significantly lower in white adipose tissue (WAT), heart, or red quadriceps at 4 weeks.

Conclusions:

  • Adult NZO mice exhibit reduced GLUT4 protein in key insulin-sensitive tissues.
  • The decline in GLUT4 in BAT of NZO mice is an early event in the progression of obesity-related metabolic dysfunction.