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Related Experiment Videos

Endotoxin affinity for provisional restorative resins

F Gagnon1, K L Knoernschild, L Payant

  • 1Department of Oral Rehabilitation, Medical College of Georgia, Augusta.

Journal of Prosthodontics : Official Journal of the American College of Prosthodontists
|December 1, 1994
PubMed
Summary

Porphyromonas gingivalis and Escherichia coli lipopolysaccharide (LPS) exhibit varying affinities for provisional resins. Resin type and surface finish influence LPS adherence and elution, with P. gingivalis LPS showing higher elution levels.

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Area of Science:

  • Dental Materials Science
  • Microbiology
  • Biomaterials

Background:

  • Provisional resins are crucial in dental restorations.
  • Bacterial endotoxins, like lipopolysaccharide (LPS), can adhere to biomaterials.
  • Understanding LPS-resin interactions is vital for infection control in dentistry.

Purpose of the Study:

  • To compare the relative affinity of Porphyromonas gingivalis and Escherichia coli lipopolysaccharide (LPS) for three provisional resins.
  • To investigate the influence of resin type and surface finish on LPS adherence and elution.

Main Methods:

  • Provisional resin discs (polymethyl methacrylate, polyethyl methacrylate) with different surface finishes were exposed to P. gingivalis or E. coli LPS.
  • LPS elution was measured over 96 hours in LPS-free water.

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  • Eluates were analyzed for LPS presence and quantity.

    • Main Results:

    • LPS adherence varied based on resin type and surface characteristics.
    • As-polymerized resins showed higher LPS elution than pumiced resins at later time points.
    • P. gingivalis LPS demonstrated greater affinity for polyethyl methacrylate, while E. coli LPS favored polymethyl methacrylate.
    • Overall, P. gingivalis LPS eluted at higher levels than E. coli LPS.

    Conclusions:

    • LPS affinity for provisional resins is determined by selective interactions.
    • Factors influencing affinity include resin chemical composition, surface finish, and LPS molecular structure.
    • These findings have implications for managing bacterial contamination on dental prosthetics.