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Polymerase chain reaction using arbitrary primer for the design and construction of a DNA probe specific for
C Ménard1, P Gosselin, J F Duhaime
1Groupe de Recherche en Ecologie buccale, Faculté de Médecine dentaire, Université Laval, Québec, Canada.
Research in Microbiology
|October 1, 1994
Abstract:
In this work, we used a novel approach for the design and construction of DNA probes which requires no knowledge of target DNA sequence. We demonstrated that species-specific genetic markers, identified as such among monomorphic, randomly amplified DNA segments generated by the polymerase chain reaction with arbitrary primer can be labelled to yield so-called "anonymous probes". We report here on the construction of such an anonymous probe, 1146 bp long, specific for the Gram-negative anaerobe Porphyromonas gingivalis, a suspected major etiologic agent of chronic periodontitis in adults.