[The effect of angiogenin on blood vessel proliferation in rats]

Human gene-engineering angiogenin, administered in methylcellulose tablets into the rat cornea induces growth of the limbus blood vessels in nanogram doses. Speed of the growth of the newly forming capillaries increases with the angiogenin dose. Pancreatic ribonuclease, highly homologous in structure with angiogenin does not induce neovascularization under the same conditions. Activation of the blood vessels proliferation under the effect of angiogenin was also observed in rat skin, but at higher doses. Angiogenin injected in 3% agarose with low melting point into abdominal skin or into the ear skin induced neovascularization in doses no less than 3 mg. Conditions, envisaging depression of the tissue immune reactions to the heterogeneous protein did not eliminate the angiogenin effect. This allows to conclude that they are caused by the direct manifestation of the angiogenic qualities of the studies growth factor.