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Improved PCR amplification/Hhal restriction for unambiguous determination of apolipoprotein E alleles
E Appel1, S Eisenberg, J Roitelman
1Institute of Lipid and Atherosclerosis Research, Sheba Medical Center, Tel Hashomer, Israel.
Clinical Chemistry
|February 1, 1995
Abstract:
We present a modification to the polymerase chain reaction amplification/Hhal restriction isotyping method for human apolipoprotein (apo) E. This method includes a mutagenic forward primer and 5'-end labeling of both primers. These modifications of the original method described by Hixon and Vernier (J Lipid Res 1990;31:545-8) allow sensitive and unambiguous determination of apoE genotypes.