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Related Experiment Videos

Murine M290 integrin expression modulated by mast cell activation

T J Smith1, L A Ducharme, S K Shaw

  • 1Department of Pathology, University of Utah School of Medicine, Salt Lake City 84132.

Immunity
|August 1, 1994
PubMed
Summary
This summary is machine-generated.

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Transforming growth factor beta (TGF-β) induces an integrin complex on intraepithelial T lymphocytes, involving the beta 7 integrin and a novel alpha chain, alpha M290. Mast cells also express this complex upon TGF-β or IgE stimulation.

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Intraepithelial T lymphocytes express an integrin complex crucial for their function.
  • This complex is known to involve the beta 7 integrin chain.

Purpose of the Study:

  • To identify and characterize the novel alpha chain associated with the beta 7 integrin in intraepithelial T lymphocytes.
  • To investigate the regulation of this integrin complex expression in different cell types.

Main Methods:

  • Antibody-based staining to detect the alpha M290 chain on mast cells.
  • Cloning of the murine homolog of the human intraepithelial T lymphocyte-alpha E gene.
  • Gene expression analysis in bone marrow-derived mast cells and TK-1 cells.

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Main Results:

  • A novel alpha chain, murine alpha M290, was identified and shown to associate with the beta 7 integrin.
  • TGF-β induced the expression of the alpha M290 chain on mast cells.
  • IgE-mediated cross-linking of Fc epsilon R1 also induced alpha M290 expression on mast cells.
  • Mast cell mediators can induce alpha M290 expression on other cells in a paracrine manner.

Conclusions:

  • The alpha M290 chain is a component of a (TGF-β)-inducible integrin complex on intraepithelial T lymphocytes.
  • Mast cells express this integrin complex upon stimulation with TGF-β or IgE.
  • Mast cell-derived factors can regulate the expression of this integrin complex on other cell types, suggesting paracrine signaling pathways.