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A PCR strategy for the isolation of glutathione S-transferases (GSTs) from nematodes
P M Brophy1, A Brown, D I Pritchard
1Department of Pharmacy, School of Applied Sciences, De Montfort University, Leicester, U.K.
International Journal for Parasitology
|November 1, 1994
Abstract:
Anchor based PCR technology has been used to isolate a GST sequence from the gastro-intestinal nematode Heligmosomoides polygyrus. A 800 base pair product was amplified from first-strand cDNA using primers based on the N-terminal sequence of purified H. polygyrus GST (upstream primer) and a non-specific polyadenylate tail with an anchor sequence (downstream primer). The product was cloned into pUC18 and sequenced. A reading frame of 648 bases in the sequence encoded a protein which has 30% homology with the alpha family of mammalian glutathione S-transferases.