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Updated: Aug 12, 2026
11:30
Analyzing the Effects of Stromal Cells on the Recruitment of Leukocytes from Flow
Published on: January 7, 2015
Secreted glucocorticoids regulate leukocyte-endothelial interactions in inflammation. A direct vital microscopic
S P Farsky1, P Sannomiya, J Garcia-Leme
1Department of Pharmacology, University of São Paulo, Brazil.
Journal of Leukocyte Biology
|March 1, 1995
Summary
Glucocorticoids suppress leukocyte interactions with blood vessel walls during inflammation. Blocking these steroids increases leukocyte rolling and migration, impacting inflammatory responses.
Area of Science:
- Immunology
- Endocrinology
- Cell Biology
Background:
- Leukocyte extravasation is crucial for inflammatory responses.
- Glucocorticoids are known to modulate immune cell behavior.
Purpose of the Study:
- To investigate the role of glucocorticoids in regulating leukocyte-endothelial interactions during inflammation.
- To elucidate the mechanisms by which glucocorticoids affect leukocyte adhesion and migration.
Main Methods:
- Adrenalectomy and metyrapone treatment to reduce endogenous glucocorticoids.
- Administration of steroid antagonists (RU 38 486) and gene expression inhibitors (actinomycin D, cycloheximide).
- Use of monoclonal antibodies against CD18 to assess its role.
Main Results:
- Reduced glucocorticoids increased leukocyte rolling and subsequent adhesion/migration.
- Steroid antagonists and gene inhibitors mimicked the effects of adrenalectomy.
- Anti-CD18 antibodies reduced adhesion and migration without affecting rolling.
Conclusions:
- Secreted glucocorticoids suppress leukocyte-endothelial interactions, limiting cell emigration during inflammation.
- This suppression involves receptor occupancy and gene expression.
- Glucocorticoids may modulate CD18 activity, influencing leukocyte migration.
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