Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Xylem-specific gene expression in loblolly pine

C A Loopstra1, R R Sederoff

  • 1Department of Forestry, North Carolina State University, Raleigh 27695-8008.

Plant Molecular Biology
|January 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sequence of the 18S-5S ribosomal gene region and the cytochrome oxidase II gene from mtDNA of Zea diploperennis.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Paternal inheritance of chloroplast DNA and maternal inheritance of mitochondrial DNA in loblolly pine.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Transient expression from microprojectile-mediated DNA transfer in pinus taeda.

Plant cell reports·2013
Same author

Targeted mapping and linkage analysis of morphological isozyme, and RAPD markers in peach.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Genetic mapping of QTLs controlling vegetative propagation in Eucalyptus grandis and E. urophylla using a pseudo-testcross strategy and RAPD markers.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Climate change and the integrity of science.

Science (New York, N.Y.)·2010
Same journal

Genetic basis of alkaloid divergence in the Solanaceae.

Plant molecular biology·2026
Same journal

Integrative approach to identify robust pattern recognition receptors in Eucalyptus grandis: novel candidates for disease resistance.

Plant molecular biology·2026
Same journal

Splicing regulation by RS2Z36 controls ovary patterning and fruit growth in tomato.

Plant molecular biology·2026
Same journal

Coumarin metabolites in Ocimum: chemical diversity, biosynthetic pathways, and network pharmacology-based prediction of multi-target anticancer potential.

Plant molecular biology·2026
Same journal

Ethanol application enhances freezing stress tolerance in Arabidopsis and sugar beet.

Plant molecular biology·2026
Same journal

CeO<sub>2</sub> Nanozymes as redox regulators: Reprogramming of reactive oxygen species (ROS) signaling for plant climate resilience.

Plant molecular biology·2026
See all related articles

Researchers identified two loblolly pine genes, PtX3H6 and PtX14A9, highly expressed in differentiating xylem. These genes may contribute to xylem-specific expression through shared regulatory sequences.

Area of Science:

  • Plant Molecular Biology
  • Forest Genetics
  • Biochemistry

Background:

  • Differentiating xylem is crucial for tree growth and wood formation.
  • Understanding gene expression in xylem is key to improving wood quality and yield.
  • Loblolly pine (Pinus taeda L.) is a significant commercial timber species.

Purpose of the Study:

  • To identify and characterize genes specifically expressed during loblolly pine xylem differentiation.
  • To investigate the potential regulatory mechanisms underlying xylem-specific gene expression.

Main Methods:

  • Cloning of genes from cDNA and genomic libraries.
  • Transcript abundance analysis in various tissues (xylem, needles, embryos, megagametophytes).
  • Bioinformatic analysis of gene sequences and promoter regions.

Related Experiment Videos

Main Results:

  • Two genes, PtX3H6 and PtX14A9, were cloned and found to be highly abundant in differentiating xylem.
  • PtX3H6 contains structural motifs similar to proline-rich cell wall proteins, and both genes show similarity to arabinogalactan proteins (AGPs).
  • A conserved 7 bp sequence in the 5' flanking regions of both genes suggests a role in vascular-specific expression, similar to known glycine-rich proteins (GRPs).

Conclusions:

  • PtX3H6 and PtX14A9 are novel genes involved in loblolly pine xylem development.
  • Their structural similarities suggest roles in cell wall composition or modification.
  • The identified cis-regulatory element may be a key factor in achieving xylem-specific gene expression in this species.