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Related Experiment Videos

Imaginal disc silencers from Ultrabithorax: evidence for Polycomb response elements

B Christen1, M Bienz

  • 1MRC Laboratory of Molecular Biology, Cambridge, UK.

Mechanisms of Development
|December 1, 1994
PubMed
Summary

Silencers of the Ultrabithorax (Ubx) gene require hunchback (hb) and Polycomb (Pc) for proper function. Their cooperation ensures stable Ubx gene silencing during development, defining specific expression domains.

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Area of Science:

  • Developmental biology
  • Gene regulation
  • Drosophila genetics

Background:

  • Silencers of the Drosophila homeotic gene Ultrabithorax (Ubx) are crucial for repressing gene activity outside its designated domain.
  • Embryonic silencing of Ubx is initiated by hunchback (hb) protein binding to silencers, defining the Ubx expression domain.

Purpose of the Study:

  • To investigate the mechanisms of Ubx gene silencing during post-embryonic development.
  • To analyze the roles of specific Ubx fragments and their combinations in imaginal disc silencing.
  • To elucidate the cooperative interactions between hunchback (hb) and Polycomb (Pc) in stable Ubx silencing.

Main Methods:

  • Examination of expression patterns conferred by individual and paired Ubx fragments in Drosophila imaginal discs.

Related Experiment Videos

  • Analysis of silencing activity mediated by specific Ubx fragments, including the BXD fragment.
  • Assessment of the requirement for hunchback (hb) and Polycomb (Pc) function in fragment-mediated silencing.

    • Main Results:

    • Ubx fragments mediating silencing in anterior imaginal disc regions contain embryonic silencers and hb target sites.
    • The BXD fragment, while not hb-dependent itself, requires combination with hb-containing fragments for silencing activity.
    • Silencing mediated by the BXD fragment also necessitates Polycomb (Pc) function, suggesting Pc or Pc-like target sites within BXD.

    Conclusions:

    • Stable silencing of the Ultrabithorax (Ubx) gene during development is achieved through the cooperative action of hunchback (hb) and Polycomb (Pc) target sites.
    • The BXD fragment's silencing activity highlights a model where distinct regulatory elements cooperate for precise gene expression control.
    • Understanding these interactions provides insights into the complex epigenetic mechanisms governing homeotic gene regulation in Drosophila.