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Determination of adenine nucleotides by fluorescence detection using high-performance liquid chromatography and

M R Stratford¹, M F Dennis

¹Gray Laboratory of the Cancer Research Campaign, Mount Vernon Hospital, Northwood, Middlesex, UK.

Journal of Chromatography. B, Biomedical Applications

|December 2, 1994

Abstract:

A novel rapid method for the analysis of adenine nucleotides in cells and tissues using post-column derivatisation with chloroacetaldehyde (CAA) followed by fluorescence detection is described. The CAA is incorporated in the eluent, but only reacts post-column when the temperature is elevated to 100 degrees C. Samples are chromatographed following neutralisation of acid extracts. Examples are given using both trichloroacetic acid extraction for cells in culture, and perchloric acid for murine liver.

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