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Micellar capillary electrophoresis separation and thermo-optical absorbance detection of products from manual peptide
1Department of Chemistry, University of Alberta, Edmonton, Canada.
Electrophoresis
|October 1, 1994
Abstract:
Micellar capillary electrophoresis is optimized for separation of phenylthiohydantoin (PTH) amino acids produced in manual Edman degradation reaction for protein sequencing. There are also two major side-products produced by the Edman degradation reaction: diphenylthiourea and dimethylphenylthiourea. We report the complete separation of 19 PTH amino acids plus the two major side-reaction products in 10 min. Capillary electrophoresis is used to identify the five residues generated by manual Edman degradation sequencing of a pentapeptide.