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Identification of protein complexes with quantitative proteomics in S. cerevisiae
Published on: March 4, 2009
Protein identifications for a Saccharomyces cerevisiae protein database
J I Garrels1, B Futcher, R Kobayashi
1Cold Spring Harbor Laboratory 11724.
Electrophoresis
|November 1, 1994
Summary
Researchers are identifying yeast proteins using 2-D gel electrophoresis and amino acid analysis to build a comprehensive protein database. This effort aids in understanding yeast gene function and regulatory pathways.
Area of Science:
- Molecular Biology
- Proteomics
- Yeast Genetics
Background:
- Understanding Saccharomyces cerevisiae gene function requires global analysis of protein expression.
- Two-dimensional (2-D) gel electrophoresis is crucial for studying protein synthesis, modification, and degradation patterns.
Purpose of the Study:
- To establish a yeast protein database by identifying spots on 2-D gels.
- To enable the study of regulatory pathways in yeast.
Main Methods:
- Utilizing protein sequencing, gene overexpression, and amino acid analysis to identify yeast proteins.
- Employing quantitative image analysis of amino acid-labeled yeast samples.
- Searching identified amino acid abundances against a database of known yeast protein sequences.
Main Results:
- Successfully identified approximately 90 proteins on yeast 2-D gel maps.
- The number of identified proteins is rapidly increasing.
- A foundation for studying regulatory pathways in normal and mutant yeast is being established.
Conclusions:
- The ongoing identification of yeast proteins via 2-D gel analysis is essential for functional genomics.
- This research facilitates the investigation of protein responses to genetic and environmental changes in yeast.
- The developed protein map will advance the study of yeast regulatory networks.
