Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

IL-4-producing CD8+ T cell clones can provide B cell help

D C Cronin1, R Stack, F W Fitch

  • 1Committee on Immunology, University of Chicago, IL 60637.

Journal of Immunology (Baltimore, Md. : 1950)
|April 1, 1995
PubMed
Summary

Certain CD8+ T cells can provide B cell help by expressing CD40 ligand, promoting B cell proliferation and antibody secretion. This interaction is crucial for immune responses, particularly when IL-4 is present.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pancreaticopleural fistula - A rare complication of pancreatitis.

Irish medical journal·2026
Same author

Faecal calprotectin as a potential biomarker of disease severity in SARS-CoV-2 infection.

The Journal of infection·2022
Same author

Acute granulomatous hepatitis in an immunosuppressed patient secondary to Q fever.

QJM : monthly journal of the Association of Physicians·2017
Same author

Miscommunicating NOTA Can Be Costly to Living Donors.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2016
Same author

CMV pneumonitis in an immunosuppressed Crohn's disease patient.

QJM : monthly journal of the Association of Physicians·2016
Same author

Renal Transplantation With Final Allocation Based on the Virtual Crossmatch.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2015

Area of Science:

  • Immunology
  • Cellular Biology
  • T cell-B cell interactions

Background:

  • CD4+ T cell interactions with B cells are vital for adaptive immunity.
  • CD40 ligand on T cells interacts with CD40 on B cells, enhancing B cell responses to cytokines like IL-4.
  • CD40 ligand expression is typically induced on CD4+ T cells.

Purpose of the Study:

  • To investigate if CD8+ T cells can also express CD40 ligand and provide help to B cells.
  • To determine the functional consequences of CD8+ T cell-derived CD40 ligand expression on B cell activation.

Main Methods:

  • Activation of IL-4-producing murine CD8+ T cell clones using anti-CD3 mAb.
  • Co-culture of activated CD8+ T cells with small resting B cells in the presence of IL-4 and/or IL-5.
  • Assessment of B cell proliferation and Ig secretion.

Related Experiment Videos

  • Inhibition studies using anti-CD40 ligand mAb.

    • Main Results:

    • Activated IL-4-producing CD8+ T cell clones expressed CD40 ligand.
    • These CD8+ T cells enhanced IL-4-driven proliferation and induced Ig secretion in B cells.
    • The B cell help provided by CD8+ T cells was dependent on CD40 ligand expression, as confirmed by mAb inhibition.

    Conclusions:

    • Certain CD8+ T cells, specifically IL-4-producing clones, can express CD40 ligand upon activation.
    • These CD8+ T cells are capable of providing functional help to B cells, including promoting proliferation and Ig secretion.
    • This finding expands the understanding of T cell help in B cell responses, highlighting a potential role for CD8+ T cells in certain immune contexts.