[Characteristics of Pseudomonas fluorescens lipopolysaccharide]

A lipopolysaccharide was isolated from Pseudomonas fluorescens IMV 1152 (biovar I). The fractions of the structural moieties of lipopolysaccharide macromolecule were extracted and studied separately. 3-hydroxydecanoic, 3-hydroxydodecanoic and 2-hydroxydodecanoic fatty acids were identified in lipid A, total quantity of those was more than 80%. In acid hydrolysate of lipid A the glucosamine and phosphoethanolamine were found. The rhamnose, arabinose, glucose, glucosamine and phosphorus were identified as the components of core oligosaccharide fractions. A trisaccharide repeating unit of the O-cpecific polysaccharide chain consists of the residues of aminosugars: 4-acet-amido-4,6-dideoxy-D-galactose, 2-acetamido-2,6-dideoxy-D-glucose and 2-acetamido-2,6-dideoxy-L-glucose. The studied lipopolysaccharide resembled the structure of lipid A and core oligosaccharide with lipopolysaccharide from type strain of Pseudomonas fluorescens IMV 4125 (ATCC 13525), whereas their O-chains were different.