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Related Experiment Videos

ADP-ribosylation and gene expression

P Zahradka1, L Yau

  • 1Department of Physiology, St. Boniface General Hospital Research Centre, University of Manitoba, Winnipeg, Canada.

Molecular and Cellular Biochemistry
|September 1, 1994
PubMed
Summary
This summary is machine-generated.

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Poly(ADP-ribose) modification does not directly regulate gene transcription initiation. However, mono(ADP-ribosyl)ation may play a role in insulin

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Cell Biology

Background:

  • Gene expression involves DNA to RNA/protein conversion.
  • Post-translational modification, including ADP-ribosylation, can affect gene expression.
  • Poly(ADP-ribose) (PAR) is involved in various cellular processes.

Purpose of the Study:

  • To investigate the role of poly(ADP-ribose) in regulating transcription initiation.
  • To explore the potential involvement of ADP-ribosylation in insulin signaling and rRNA synthesis.

Main Methods:

  • Utilized cell-free systems to study transcription initiation of ribosomal RNA and proteins.
  • Assessed the impact of poly(ADP-ribose) polymerase presence and activity on transcription.
  • Administered 3-aminobenzamide to insulin-treated hepatoma cells to observe effects on rRNA synthesis.

Related Experiment Videos

Main Results:

  • Poly(ADP-ribose) polymerase presence or activity did not affect transcription initiation in cell-free systems.
  • No direct link between poly(ADP-ribosyl)ation and gene transcription was detected.
  • 3-aminobenzamide inhibited insulin-stimulated rRNA synthesis in hepatoma cells at high concentrations.

Conclusions:

  • Poly(ADP-ribosyl)ation does not appear to directly regulate gene transcription initiation.
  • Mono(ADP-ribosyl)ation may be involved in the cellular action of insulin.
  • A potential role for mono(ADP-ribosyl)ation in insulin-induced rRNA gene expression signal transduction is proposed.