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Use of firefly luciferase gene for plasmid copy number determination
C Coronado1, M E Vázquez, A Cebolla
1Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Sevilla, Spain.
Plasmid
|November 1, 1994
Abstract:
A simple and rapid method for determining plasmid copy number is described. The eukaryotic luc gene is used as a marker to tag plasmid derivatives of several well-known vectors, and by measuring light activity plasmid copy number is determined. A comparative analysis using a standard hybridization procedure to estimate plasmid copy number by densitometry is also described.