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Inducible accessory function of a macrophage cell line
F B Aiello1, L Gusella, D L Longo
1Biological Carcinogenesis and Development Program, Program Resources Inc./DynCorp, NCI-Frederick Cancer Research and Development Center, MD.
Immunopharmacology and Immunotoxicology
|August 1, 1993
Summary
Antigen-presenting cells (APCs) require costimulatory molecules for T-cell proliferation. This study identifies a macrophage cell line (FLJ2) that gains antigen-presenting function upon activation, highlighting the role of inducible molecules and cellular adhesion.
Area of Science:
- Immunology
- Cell Biology
Background:
- T-cell proliferation requires both T-cell antigen receptor (TCR) occupancy and costimulatory signals.
- Costimulatory molecules are typically not constitutively expressed on antigen-presenting cell (APC) surfaces.
Purpose of the Study:
- To investigate a cloned macrophage cell line (FLJ2) for inducible antigen-presenting cell (APC) function.
- To identify the molecules and mechanisms involved in APC activation.
Main Methods:
- Utilized a cloned macrophage cell line (FLJ2).
- Activated FLJ2 cells with interferon-gamma (IFN-γ) or lipopolysaccharide (LPS).
- Assessed T-cell proliferation and antigen presentation.
- Analyzed expression of molecules like ICAM-1 and LFA-1.
- Tested the effect of anti-LFA-1 antibody.
Main Results:
- Unactivated FLJ2 cells did not induce T-cell proliferation.
- Activated FLJ2 cells presented alloantigen and stimulated T-cell proliferation.
- APC function was preserved in fixed, activated FLJ2 cells.
- Inducible molecules were distinct from Ia and IL-1.
- ICAM-1 and LFA-1 expression increased upon activation.
- Anti-LFA-1 antibody blocked APC function.
Conclusions:
- FLJ2 exhibits inducible APC function upon activation with IFN-γ or LPS.
- Increased expression of ICAM-1 and LFA-1, and their role in blocking APC function, suggests improved cellular adhesion is critical for activation.
- This highlights the importance of inducible costimulatory molecules and cell adhesion in APC function.