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Two early replicated, developmentally controlled genes of Physarum display different patterns of DNA replication by

J D Diller1, H W Sauer

  • 1Department of Biology, Texas A&M University, College Station 77843.

Chromosoma
|September 1, 1993
PubMed

Insights

Physarum DNA replication origins vary. One gene has a single origin, while another has multiple, inefficient origins where replication forks meet and persist. This reveals diverse replication strategies in eukaryotes.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Eukaryotic replication origin characterization is limited to select model organisms.
  • Understanding DNA replication origins is crucial for comprehending genome stability and inheritance.

Purpose of the Study:

  • To investigate the nature and usage of replication origins in the myxomycete Physarum.
  • To analyze the replication patterns of two developmentally regulated, early-replicating genes.

Main Methods:

  • Utilized highly synchronous Physarum plasmodia cultures.
  • Employed two-dimensional agarose gel electrophoresis for replication analysis.
  • Analyzed restriction fragment length polymorphisms (RFLPs) for allelic coordination.

Main Results:

  • Identified a single, discrete replication origin near the LAV1-5 gene.
  • Discovered multiple, inefficient origins within a 15 kb domain of the LAV1-2 gene.
  • Observed persistent replication fork collision structures within the LAV1-2 domain.

Conclusions:

  • Physarum exhibits diverse DNA replication origin strategies, including both fixed narrow origins and broader zones with multiple origins.
  • Replication of alleles on different parental chromosomes is highly coordinated.
  • The findings contribute to the understanding of eukaryotic DNA replication mechanisms.

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