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DNA amplification fingerprinting using arbitrary oligonucleotide primers
G Caetano-Anollés1, B J Bassam
1Institute of Agriculture, University of Tennessee, Knoxville 37901-1071.
Applied Biochemistry and Biotechnology
|August 1, 1993
Summary
DNA amplification fingerprinting (DAF) uses short DNA primers to generate unique genetic profiles for identification and mapping. This method provides detailed and reproducible DNA patterns suitable for genetic markers and fingerprinting applications.
Area of Science:
- Molecular Biology
- Genetics
- Biotechnology
Background:
- DNA amplification fingerprinting (DAF) is a technique for genetic analysis.
- It utilizes short, arbitrary oligonucleotide primers for DNA profiling.
Purpose of the Study:
- To describe DNA amplification fingerprinting (DAF) as a method for genetic typing and mapping.
- To highlight its utility in generating genetic markers and DNA fingerprints.
Main Methods:
- Enzymatic amplification of DNA using one or more short arbitrary oligonucleotides (> or = 5 nt).
- Analysis of amplified DNA fragments using polyacrylamide gel electrophoresis and silver staining.
Main Results:
- DAF generates a spectrum of DNA products characteristic of the DNA template.
- Simple banding patterns serve as genetic markers, while complex patterns are suitable for DNA fingerprinting.
- Polyacrylamide gel electrophoresis and silver staining provide detailed and reproducible DAF patterns.
Conclusions:
- DAF is a versatile technique for genetic typing and mapping.
- The method produces reproducible and informative DNA profiles.
- It is applicable for both genetic marker identification and DNA fingerprinting.