Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cyclic-GMP-mediated decrease in permeability of human umbilical and pulmonary artery endothelial cell monolayers

R G Westendorp1, R Draijer, A E Meinders

  • 1Gaubius Laboratory, IVVO-TNO, Leiden, The Netherlands.

Journal of Vascular Research
|January 1, 1994
PubMed
Summary

Cyclic guanosine monophosphate (cGMP) and cyclic adenosine monophosphate (cAMP) regulate endothelial permeability. Both signaling molecules, particularly cGMP, can reduce vascular leakage and improve endothelial barrier function, offering potential therapeutic targets.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ultrafine particle exposure for bicycle commutes in rush and non-rush hour traffic: A repeated measures study in Copenhagen, Denmark.

Environmental pollution (Barking, Essex : 1987)·2021
Same author

Exposure to ultrafine particles while walking or bicycling during COVID-19 closures: A repeated measures study in Copenhagen, Denmark.

The Science of the total environment·2021
Same author

HDL functionality in South Asians as compared to white Caucasians.

Nutrition, metabolism, and cardiovascular diseases : NMCD·2016
Same author

Cardiovascular flexibility in middle-aged overweight South Asians vs. white Caucasians: response to short-term caloric restriction.

Nutrition, metabolism, and cardiovascular diseases : NMCD·2015
Same author

Statins in type 2 diabetes mellitus: target on lipids and vascular wall function.

Netherlands heart journal : monthly journal of the Netherlands Society of Cardiology and the Netherlands Heart Foundation·2015
Same author

Diagnostic criteria for sarcopenia and physical performance.

Age (Dordrecht, Netherlands)·2013

Area of Science:

  • Vascular Biology
  • Cellular Physiology
  • Biochemistry

Background:

  • Endothelial cell contraction is crucial for vascular leakage.
  • Elevated cyclic adenosine monophosphate (cAMP) improves endothelial barrier function.
  • Cyclic guanosine monophosphate (cGMP) also influences smooth muscle contraction.

Purpose of the Study:

  • To compare the roles of cAMP and cGMP in regulating human endothelial cell permeability.
  • To investigate the effects of ANP, SNP, and 8-bromo-cGMP on endothelial barrier function.

Main Methods:

  • Cultured human endothelial cell monolayers were used.
  • Measurements of permeability, transendothelial electrical resistance, and intracellular cGMP/cAMP levels were performed.
  • Agents like atrial natriuretic peptide (ANP), sodium nitroprusside (SNP), forskolin, and NG-nitro-L-arginine methyl ester were applied.

Related Experiment Videos

Main Results:

  • cGMP elevation via ANP or SNP, or direct addition of 8-bromo-cGMP, suppressed thrombin-induced permeability increase.
  • Inhibition of nitric oxide production increased permeability, an effect reversed by 8-bromo-cGMP or ANP.
  • Increased cAMP (via forskolin) reduced permeability under basal and thrombin-stimulated conditions.

Conclusions:

  • Both cAMP and cGMP are potent regulators of endothelial permeability.
  • cGMP, in addition to cAMP, fine-tunes endothelial barrier function.
  • These findings suggest potential therapeutic strategies targeting these cyclic nucleotides to control vascular leakage.