Related Experiment Videos
Temporal changes in rabbit urinary bladder function and DNA synthesis during chronic treatment with furosemide
T L Tammela1, R M Levin, F C Monson
1Division of Urology, University of Pennsylvania, Philadelphia.
The Journal of Urology
|February 1, 1994
Summary
Furosemide-induced diuresis in rabbits increased bladder mass and contractile function. These changes, including increased bladder mass and improved contractility, represent a beneficial physiological response to increased urine production.
Area of Science:
- Urology
- Pharmacology
- Physiology
Background:
- Diuresis, or increased urine production, can impact bladder function.
- Previous studies indicated furosemide increases bladder mass and contractility.
Purpose of the Study:
- To investigate the temporal effects of furosemide-induced diuresis on rabbit bladder function.
- To assess changes in micturition, bladder mass, proliferation, DNA synthesis, and contractility over time.
Main Methods:
- Male New Zealand White rabbits received furosemide via osmotic pumps.
- Micturition, bladder mass, DNA synthesis (3H-thymidine uptake), and contractile responses were measured at 3, 7, and 14 days.
Main Results:
- Furosemide induced polyuria and polydipsia within 1 day, persisting for 7 days.
- Bladder mass significantly increased at 3 and 7 days.
- Bladder DNA synthesis and concentration increased at 3 days but normalized by 7 days.
- Contractile responses to stimulation and agonists were enhanced at 7 days, not 3 days.
Conclusions:
- Modest increases in bladder mass are a normal physiological response to diuresis.
- Enhanced bladder contractility follows the increase in bladder mass.
- These adaptations appear to be beneficial responses to sustained diuresis.