Related Experiment Videos
Interferon-gamma induces dipeptidylpeptidase IV expression in human glomerular epithelial cells
V Stefanovic1, N Ardaillou, P Vlahovic
1INSERM 64, Hôpital Tenon, Paris, France.
Immunology
|November 1, 1993
Summary
Dipeptidylpeptidase IV (DPP IV) is expressed on kidney cells and its levels increase with interferon-gamma (IFN-gamma) treatment. This suggests IFN-gamma may up-regulate DPP IV in kidney diseases and transplant rejection.
Area of Science:
- Nephrology
- Immunology
- Molecular Biology
Background:
- Dipeptidylpeptidase IV (DPP IV) is present on glomerular visceral epithelial cells and has immunogenic properties.
- Anti-DPP IV antibodies can induce glomerulonephritis, suggesting DPP IV's role in kidney disease.
Purpose of the Study:
- To investigate the expression and regulation of DPP IV in cultured human glomerular visceral epithelial cells.
- To understand how interferon-gamma (IFN-gamma) affects DPP IV expression.
Main Methods:
- Cell culture of human glomerular visceral epithelial cells.
- Enzyme activity assays and immunofluorescence staining.
- Northern blot analysis to assess DPP IV mRNA levels.
Main Results:
- DPP IV was confirmed as an alkaline ectoenzyme in glomerular epithelial cells.
- Interferon-gamma (IFN-gamma) significantly induced DPP IV expression and mRNA levels in a time- and dose-dependent manner.
- Interleukin-1 and cyclic AMP showed minor stimulatory effects, while other agents were ineffective.
Conclusions:
- DPP IV expression in glomerular epithelial cells can be upregulated by IFN-gamma, potentially from activated T lymphocytes.
- This upregulation may contribute to kidney diseases and lymphocyte-mediated graft rejection.