Related Experiment Videos
Identification of a second poly(A) polymerase in Escherichia coli
M P Kalapos1, G J Cao, S R Kushner
1Boston Biomedical Research Institute, MA 02114.
Biochemical and Biophysical Research Communications
|January 28, 1994
Abstract:
A second poly(A) polymerase (PAP II) has been identified in Escherichia coli using a strain carrying a deletion of pcnB (the structural gene for PAP I; Cao and Sarkar, 1992b) and pnp-7 (a null mutation in the structural gene for polynucleotide phosphorylase). While PAP I has a M(r) of 53,000, PAP II is a smaller protein with a native M(r)-35,000. PAP II differs from PAP I in preferring poly(A) over tRNA primers and being more thermolabile. The presence of multiple poly(A) polymerases in E. coli raises interesting questions regarding the role of polyadenylation in mRNA synthesis and decay.