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HNF1 alpha activates the aminopeptidase N promoter in intestinal (Caco-2) cells
J Olsen1, L Laustsen, J Troelsen
1Department of Medical Biochemistry, Panum Institute, University of Copenhagen, Denmark.
FEBS Letters
|April 11, 1994
Abstract:
The importance of HNF1 binding proteins for intestinal aminopeptidase N expression was investigated using the Caco-2 cell-line. Aminopeptidase N promoter activity in Caco-2 cells depends on the HNF1 element (positions -85 to -58) and co-transfection with an HNF1 alpha expression vector demonstrates a direct activation of the promoter by HNF1 alpha through this element. Electrophoretic mobility shift assays using nuclear extracts from Caco-2 cells show the presence of high amounts of HNF1 binding proteins irrespective of their state of differentiation.