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Keratinocyte transglutaminase expression varies in squamous cell carcinomas
M Duvic1, D C Nelson, M Annarella
1Department of Dermatology, University of Texas Medical School at Houston 77030.
The Journal of Investigative Dermatology
|April 1, 1994
Summary
Type I transglutaminase (TGase I) protein and mRNA expression varies in squamous cell carcinomas. Differentiation influences TGase I gene regulation, suggesting a role in tumor development.
Area of Science:
- Biochemistry
- Molecular Biology
- Dermatology
Background:
- Type I transglutaminase (TGase I) is a protein abundant in keratinocytes and implicated in skin disorders like psoriasis.
- TGase I plays a role in epidermal differentiation and keratinocyte structure.
Purpose of the Study:
- To investigate the expression of TGase I protein and mRNA in squamous cell carcinomas (SCCs).
- To explore the relationship between TGase I expression, tumor differentiation, and gene regulation in SCCs.
Main Methods:
- Immunohistochemistry and Western blotting to detect TGase I protein.
- Reverse transcriptase-polymerase chain reaction (RT-PCR) to quantify TGase I mRNA levels.
- Analysis of TGase I promoter and genomic DNA for regulatory elements and methylation differences.
Main Results:
- TGase I protein was detected in well-differentiated, skin-derived SCCs, with expression levels varying significantly compared to keratinocytes.
- Protein expression correlated with mRNA levels in most SCC lines.
- Unique DNA fragments and potential methylation differences in the TGase I promoter were observed in specific SCC lines.
Conclusions:
- Transcriptional control of TGase I gene expression in SCCs is influenced by promoter cis elements.
- The degree of tumor squamous differentiation impacts TGase I gene regulation.
- These findings suggest TGase I's role in squamous carcinoma development and differentiation.