Related Experiment Videos
Cord blood banking for stem cell transplant
F Bertolini1, L Lazzari, C Corsini
1Division of Hematology, Maggiore Hospital, Milano, Italy.
The International Journal of Artificial Organs
|December 1, 1993
Summary
Umbilical cord blood collection is feasible for unrelated transplants. A rapid clamping technique yielded sufficient hematopoietic progenitor cells for potential adult engraftment.
Area of Science:
- Hematology
- Transplantation Medicine
- Biotechnology
Background:
- Umbilical cord blood (UCB) is a valuable source of hematopoietic progenitor cells (HPCs).
- UCB transplantation is established for pediatric patients.
- The feasibility of UCB banking for unrelated adult transplants requires evaluation.
Purpose of the Study:
- To assess the viability of establishing a cord blood bank for unrelated transplants.
- To determine the quantity and quality of HPCs collected using a specific cord clamping protocol.
Main Methods:
- Umbilical cords were clamped within 20 seconds of delivery.
- Volume of collected cord blood was measured.
- Colony-forming unit-granulocyte-macrophage (CFU-GM) counts were determined to assess HPC content.
Main Results:
- An average of 86 +/- 25 ml of cord blood was collected per unit.
- Over 53% of collected samples contained sufficient CFU-GM for potential engraftment in adult recipients (50-70 kg).
- The rapid clamping method facilitated adequate HPC collection.
Conclusions:
- Establishing a cord blood bank for unrelated transplants is feasible.
- The described collection method yields sufficient HPCs for potential adult transplantation.
- Further research into optimizing UCB banking protocols is warranted.