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Site selectivity in the glycation of alpha A- and alpha B-crystallins by glucose
E C Abraham1, M Cherian, J B Smith
1Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta 30912.
Biochemical and Biophysical Research Communications
|June 30, 1994
Abstract:
We determined the site selectivity of glycation by glucose (glucosylation) in alpha A- and alpha B-crystallins using two independent approaches. HPLC purified 14C-glucose labeled chymotryptic peptides and affinity chromatography/HPLC purified fully glycated peptides were identified by FAB-MS. Lys 11 and 78 of alpha A-crystallin and Lys 90 and/or 92 of alpha B-crystallin were the fast reacting sites of glucosylation.